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PCR Cloning Kit From Invitrogen for Sequencing of PCR Products
(1)
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Thermo Fisher Scientific
February 08, 2024
I effectively employed the TOPO TA Cloning Kits to insert Taq polymerase directly–amplified PCR products into a sequencing-ready plasmid ...
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Ki-67 Antibody From Thermo Fisher Was Used as a Marker of Cell Proliferation After DMAG-17 Chemotherapy Treatment.
(1)
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Thermo Fisher Scientific
February 08, 2024
Activation of endogenous retrotransposons frequently occurs in cancer cells and contributes to tumor genomic instability. To test whether ...
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Nice Mouse IL-2 IS From Miltenyi Biotec for T-Cell Activation
(1)
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Miltenyi Biotec
February 07, 2024
IL-2 plays an important role in the activation and maintenance of immune responses and in lymphocyte development. We used IL-2 IS (improved ...
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Superb SYBR Green Master Mix From Thermo Fisher Scientific
(1)
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Thermo Fisher Scientific
February 07, 2024
We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.
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GFP Antibody From Cell Signaling Technology Produces Clear Bands
(1)
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Cell Signaling Technology
February 07, 2024
The performance of this antibody (GFP) in Western blots has been consistently reliable without any issues. Employing a 1:1000 dilution in ...
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Efficient and Specific cDNA Synthesis Can be Achieved With the First-Strand Synthesis System From Invitrogen
(1)
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Thermo Fisher Scientific
February 07, 2024
I used the SuperScript III First-Strand Synthesis System for RT-PCR because it provides efficient cDNA synthesis from both poly(A)+ and ...
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An Average Anti-Lambda Monoclonal Antibody
(1)
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BD Biosciences
February 07, 2024
Monoclonal anti-kappa and anti-lambda antibodies are not always of good quality. This is why many labs use polyclonal antibodies instead. ...
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Anti-Mouse CD64 Antibody From BioLegend Produces Good Results for the Characterization of Macrophages
(1)
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BioLegend
February 07, 2024
This antibody gives good results for the characterization of macrophages in LPMCs by flow cytometry.
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Anti-HA Matrix From Roche
(1)
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Roche
February 06, 2024
I have consistently relied on the Anti-HA High-Affinity antibody (clone 3F10) for its exceptional specificity in recognizing the YPYDVPDYA ...
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Easy and Solid CD10 Staining With Antibody From BD Biosciences
(1)
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BD Biosciences
February 06, 2024
CD10 is positive on mature neutrophils, but can also be seen on immature B cells (hematogones). We needed a good CD10 antibody that would ...
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Highly Recommended Anti-Mouse CD31 Antibody From BioLegend for Flow
(1)
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BioLegend
February 06, 2024
I use this antibody to stain for lymphatic endothelial cells in the mouse lymph node and it works well.
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HSP70 Expression After Anti-Cancer Treatment
(1)
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Enzo Life Sciences, Inc.
February 06, 2024
Activation of endogenous retrotransposons frequently occurs in cancer cells and contributes to tumor genomic instability. To test whether ...
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Well Working Secondary Antibody From Thermo Fisher for Immunofluorescence Detection (405 nm laser)
(1)
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Thermo Fisher Scientific
February 06, 2024
This secondary anti-mouse antibody from Thermo Fisher Scientific (Invitrogen) is a good choice for the detection of primary mouse antibodies...
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Cost Effective Product with Excellent Staining Efficacy From BD Biosciences
(1)
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BD Biosciences
February 06, 2024
This buffer was used for cell cycle staining for oesophageal adenocarcinoma cell lines. As opposed to other PI stains that need overnight ...
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Great ACK Lysing Buffer From Gibco for Red Blood Cell Lysis
(1)
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Thermo Fisher Scientific
February 05, 2024
We have been using this ACK lysing buffer for several projects. Whenever we need to isolate single cells from mouse organs, we will use this...
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Mini Protein Gels From Invitrogen for Western Blot
(1)
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Thermo Fisher Scientific
February 05, 2024
We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.
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Anti-IRF3 Antibody From Abcam Produces Great results for Western Blot
(1)
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Abcam
February 05, 2024
In the SH-SY5Y cell study involving beta-amyloid or isolated mitochondria treatment, the IRF-3 antibody (#ab50772) consistently demonstrated...
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High Quality IL-7 Cytokine From R&D Systems for Supporting T-cell expansion
(1)
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R&D Systems, a Bio-Techne Brand
February 05, 2024
I use recombinant human IL-7 protein to expand my human T cells following transduction. This cytokine works great in supporting expansion ...
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This IL-15 Cytokine Works Great to Support T-Cell Growth and Expansion
(1)
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R&D Systems, a Bio-Techne Brand
February 05, 2024
Along with IL-7, I use this IL-15 from R&D Systems to supplement my media when culturing my human T cells.
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Clear Signal Was Obtained with CD5 PE From BD Biosciences
(1)
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BD Biosciences
February 05, 2024
CD5 is used for identification of T cells, but also in the diagnosis of CLL, in which B cells express CD5 aberrantly. Typically, PE is too ...
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Easy to Use Mouse IL-6 ELISA Kit from Abcam
(1)
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Abcam
February 02, 2024
We used an IL-6 ELISA kit from Abcam to assess mice's immunoreactivity by quantifying IL-6 levels in the blood plasma after viral-mimetic ...
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Highly Recommended Kit for PCR Purification
(1)
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February 02, 2024
I aim to clone gene fragments by PCR for doing genetic knockout. Therefore, I used this kit to purify my PCR products, such as removing ...
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Ideal Perm/Wash Buffer for Intracellular Cytokine Staining
(1)
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BD Biosciences
February 02, 2024
We have been using this BD Perm/Wash Buffer in flow cytometry analysis. It can be used to permeabilize the cells and do intracellular ...
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Endo-Free Plasmid Maxi Kit From Omega Biotek Works Perfectly
(1)
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Omega Bio-Tek, Inc.
February 02, 2024
We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.
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Phospho-IRF Antibody From Cell Signaling Technology Works Well for Western Blot
(1)
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Cell Signaling Technology
February 02, 2024
In the SH-SY5Y cell study with beta-amyloid or isolated mitochondria treatment, the pIRF-3 antibody (#4947S) consistently exhibited ...
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Brilliant Experience With the Luciferase Reporter Assay System From Promega
(1)
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Promega
February 02, 2024
I used the dual luciferase assay to measure transcriptional activation using a Gal4 synthetic transcription assay. I co-transfected the GAL4...
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Ideal Antibody From Biolegend for Lymphatic Endothelial Cell Staining
(1)
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BioLegend
February 02, 2024
This antibody stains CD45-negative cells well! I see good separation in my populations as podoplanin labels lymphatic endothelial cells.
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Bright Anti-Mouse Siglec-F Antibody
(1)
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BD Biosciences
February 02, 2024
This Siglec-F antibody gives a reliable signal for the identification or exclusion of Eosinophils in PMBC/LPMC.
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DRP1 Rabbit mAb From Cell Signaling Has Good Signal
(1)
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Cell Signaling Technology
January 29, 2024
The goal of our research is to study the drug treatment effect on mitochondrial dynamics. Dynamin-related protein 1 (DRP1) affects ...
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KAPA LongRange Kit From Roche Produces High Quality Results
(1)
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Roche
January 29, 2024
We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.
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Cryo Mold From Tissue-Tek for Uniform Tissue Sectioning
(1)
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January 26, 2024
The major focus of our research is to determine the significance of tubuloglomerular feedback (TGF) responsiveness in physiological and ...
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Tissue Embedding Medium From Fisher Scientific Doesn't Leave Residues
(1)
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Fisher Scientific
January 26, 2024
Our lab studies acute and chronic kidney disorders in rodents and one of our areas of interest is immunohistochemistry staining. We use this...
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Oct-4 Antibody Worked Great for ICC on iPSCs
(1)
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Cell Signaling Technology
January 26, 2024
I used this antibody at 1:200 dilution to confirm OCT-4 expression in my iPSC cultures. Worked great and did not have much background. I ...
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This Antibody From BioLegend Clearly Stains CD8a+ T Cells in Primary Cells
(1)
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BioLegend
January 26, 2024
I regularly use this antibody in my basic immune cell panel to identify CD8a subpopulations within my CD3+ T cells. Especially because it is...
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Phospho-TBK1/NAK Antibody From Cell Signaling Produces Clear Bands
(1)
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Cell Signaling Technology
January 26, 2024
In the investigation involving SH-SY5Y cells subjected to beta-amyloid treatment and/or exposure to isolated mitochondria, the reliability ...
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TBK1/NAK From Cell Signaling Shows Excellent Expression for Western Blot
(1)
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Cell Signaling Technology
January 26, 2024
In the study involving SH-SY5Y cells treated with beta-amyloid and/or exposed to isolated mitochondria, the TBK1/NAK antibody (#51872S) ...
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Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit From Thermo Fisher is Better Than BrdU
(1)
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Thermo Fisher Scientific
January 25, 2024
The Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in ...
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Oxphos Antibody Cocktail for Assessing the Mitochondria State in NPAS3 Model
(1)
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Thermo Fisher Scientific
January 25, 2024
This product is a cocktail of multiple OxPhos antibodies. We used this kit for Western Blotting analysis of the relative levels of the 5 ...
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Rapid Assay From Promega for Detecting Lactate Secretion
(1)
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Promega
January 25, 2024
We measure cell culture lactate (secreted extracellular lactate) to assess energy metabolism changes, specifically glycolysis, in cultured ...
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Superb MOR-1 Antibody From Santa Cruz
(1)
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Santa Cruz Biotechnology, Inc.
January 25, 2024
MOR-1 antibody from Santa Cruz was used to evaluate the mu opioid receptor (MOR) protein expression in isolated mouse astrocytes.
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Bright and Specific S62-74 Epitope CD4+ T cell Tetramer for Identification of Spike Protein
(1)
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January 25, 2024
The PE conjugated S62 Tetramer has been working really well for identifying CD4+ T cells that respond to the ancestral Spike protein from ...
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LS BIO Human NRGN ELISA Kit's Performance in New Application
(1)
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LSBio
January 25, 2024
The ELISA kit for human neurogranin is intended for samples such as plasma, serum, and tissue homogenates. However, we used the kit for ...
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High Quality Total RNA Isolation With the RNeasy Mini Kit From Qiagen
(1)
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January 24, 2024
The major focus of our research is to determine the significance of tubuloglomerular feedback (TGF) responsiveness in physiological and ...
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Ideal Actin Monoclonal Antibody for Western Blot
(1)
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Thermo Fisher Scientific
January 24, 2024
By consistently using the stable and species-specific actin antibody as my loading control, I ensure accurate protein analysis in Western ...
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Mouse Monoclonal Anti-Human IgG From Abcam Gives Bright Staining
(1)
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Abcam
January 24, 2024
We are interested in studying the secretion of immunoglobulins from multiple myeloma cell lines, thus the detection of them is of importance...
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Mutagenesis Kit From New England Biolabs Enables Rapid, Site-Specific Mutagenesis of Double-Stranded Plasmid DNA
(1)
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New England Biolabs
January 24, 2024
The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit ...
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Highly Recommended DYKDDDDK Tag Monoclonal Antibody (5A8E5) From GenScript
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Genescript
January 24, 2024
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. The DYKDDDDK peptide is a ...
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Beta Tubulin Loading Control Monoclonal Antibody With Minimal Cross-Reactivity From Invitrogen
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Thermo Fisher Scientific
January 24, 2024
As a researcher, I find Western blotting to be a powerful technique for investigating protein stability, interactions, and expression levels...
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Excellent Cell Culture Microplates From Agilent
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Agilent Technologies
January 23, 2024
Mammalian cell culture is an important tool for research, clinical, and pharmaceutical applications. Cells isolated from animal tissues can ...
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Decent Anti-Mouse CD19 Antibody From BioLegend
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BioLegend
January 23, 2024
This antibody provides a good CD19 mouse staining on lymphocytes and an acceptable background. It allows good detection of mouse CD19-...
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