This Antibody Works Much Better Than Anti-CD3 to Identify Mouse T cells via Flow Cytometry

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Anti-mouse TCRb in BUV737

Catalog Number:

612821

Clone Number:

H57-597

I use this antibody to identify T cells via flow cytometry in mouse lymph nodes. I like it as the separation between positive and negative populations is much more clear than the anti-CD3 antibodies I've tried.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse lymphocytes

Primary Incubation

1:500, 30 min, 4 degrees C

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This product helps me identify and gate out T cells via flow cytometry in mouse lymph nodes. I see very good separation which is markedly better than the previous anti-CD3 antibody I was using to distinguish T cells from non-T immune cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great separation between positive and negative populations.

The Bad

Nothing bad about this.

The Bottom Line

Use this antibody to identify mouse T cells via flow cytometry! Works amazing.

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