Highly Recommended CD45R Flow Cytometry Antibody From BD Biosciences

February 29, 2024

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

anti-mouse CD45R (B220) in BUV496

Catalog Number:

612950

Clone Number:

RA3-6B2

Info:Supplier Page 

Browse Similar Products: CD45R Flow Cytometry Antibody Products

I use this antibody to distinguish mouse B cells from other immune populations. It works well in mouse secondary lymphoid organs like the spleen and lymph nodes.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse lymphocytes

Primary Incubation

1:500, 30 min, 4 degrees C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This product helps distinguish mouse B cells versus non-B cell immune cells in the lymph nodes via flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Can get fairly good separation with very little antibody.

The Bad

None

The Bottom Line

I would recommend this antibody to distinguish mouse B cells in the lymph node via flow cytometry.

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