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PE anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand) Antibody
107405
HK5.3
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The PE iCos Ligand antibody works well for identifying iCos Ligand expression on myeloid immune cells from cell populations isolated from mouse lungs and bronchoalveolar lavage fluid. I found this antibody to provide clear shifts at 1:100-1:200 dilution.
Flow cytometry
Mouse BALF, Lungs
1:100, 30 mins at 4 degrees C in the dark
Fc Shield
BD cytofix/cytoperm; 100 ul for 20 mins at 4 degrees C
None
5 laser Aurora Flow cytometer
I used this product to identify shifts in iCos ligand expression on dendritic cells, alveolar macrophages and monocytes isolated from the lung of unimmunized versus intranasally immunized mice. Cells were isolated from BALF using 1 ml of 1% BSA in PBS and from lungs through collagenase digestion; RBC lysis was performed using ACK buffer. Cells were then stained with viability dye followed by extra-cellular antibodies such as CD45, CD90.2, CD19, Siglec-F, CD11b, CD11c, Ly6C, Gr1, MHCII, MHCI. I have not seen any spillover issues with this antibody and fluorophore combination.
N/A
Specific, bright, and versatile for numerous panels.
Dilution of 1:100.
I would highly recommend the PE ICos Ligand antibody for identifying shifts in co-stimulatory molecule expression in cells.