BD Biosciences
BD™ CD30 PE
341635
Ber-H83
Info:Supplier Page
Browse Similar Products: Anti-CD30 Antibody Products
CD30 is never easy to detect by flow cytometry! This PE-conjugate is the best one I have found. It is not easy to optimize because it is really hard to get cells that are CD30+. But this antibody performs well.
Flow cytometry
Blood and/or bone marrow
15-20 minutes in the fridge
5% FBS
None
Flow Cytometer equipped with Blue laser
The results displayed in the image show a nice negative population (normal lymphocytes), and a population of CD30+ cells that are clearly higher on side scatter. These are Reed-Sternberg cells identified in the setting of Hodgkin Lymphoma.
N/A
Specific antibody. Bright fluorochrome.
Hard to optimize. May need to use 20 uL per test.
This is an antibody that your lab will probably use infrequently, but it works! Therefore, it is worth the investment.