Bag-3 Antibody From Santa Cruz Biotechnology is Perfect for the Detection of YTS NK Cells

February 27, 2024

Center For Applied Genomics
Childrens Hospital Of Philadelphia
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Bag-3 (19) Antibody

Catalog Number:

sc-136467

Clone Number:

Bag-3 (19)

BAG-3 mouse monoclonal antibody detects Bag-3 in the YTS NK cell line and CLEC16A over expressing YTS NK cells. The antibody detects a protein band of 74kDa at a dilution of 1:1000.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

YTS NK cell line

Primary Incubation

1 hr, 1;1000 dilution, room temperature

Blocking Agent

3% BSA in TBST

Secondary Incubation

1 hr, 1:10000, room temperature

Tertiary Incubation

Not applicable

Detection

Bound antibody was detected with WesternBright ECL kit (Advansta).

Results Summary

Detects BAG3 expression YTS and Clec16a over expressing NK cells. Was looking to find out if CLEC16a interacts with BAG-3.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody detects BAG-3.

The Bad

The antibody works great.

The Bottom Line

Antibody detects BAG-3 in YTS NK cells. Highly recommended.

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