Excellent and Super Bright Anti-Mouse MHC II From BioLegend

February 26, 2024

WCM
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-MHCII mouse

Catalog Number:

107614

Clone Number:

This super bright MHCII antibody provides bright staining on LPMCs for the detection of cDC1 in a standard myeloid immunophenotyping panel or MHCII expression on other APCs. Highly recommended.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

LPMC

Primary Incubation

1:400 in FACS buffer, 20 min on ICE

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

This super bright MHCII antibody provides a bright staining on LPMCs for the detection of cDC1 (as shown here) in a standard myeloid immunophenotyping panel or MHCII expression on other APCs. Highly recommended. Dilute at least 1:400-1:500 and/or adjust laser since it's really bright.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Super bright, little will go a long way.

The Bad

Nothing.

The Bottom Line

Great antibody, highly recommended.

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