Invitrogen
Lipofectamine 3000 Transfection Reagent
L3000001
Info:Supplier Page
I often transiently overexpress proteins in U2OS cells and found Lipofectamine 3000 to be the best transfection reagent for this cell line.
Transfection
Cells
Follow manufacturer's protocol.
The image shows good expression of a nuclear protein in U2OS cells when the plasmid was transfected using lipofectamine 3000.
N/A
Low cell death.
None.
Good transfection reagent with less toxicity.