Abcam
Mouse monoclonal Anti-Human IgG Fc (PE)
ab99761
JDC-10
Info:Supplier Page View Product Specs
Browse Similar Products: Anti-Human Anti-IgG Antibody Products
We are interested in studying the secretion of immunoglobulins from multiple myeloma cell lines, thus the detection of them is of importance. Thus, we commonly use this antibody for cell line characterization.
Flow cytometry
Cell lines
1:50, 20 min, 4 degrees C
N/A
BD LSR II
150.000 cells from an IgG- and an IgG+ cell line were stained with this antibody (intracellular staining). A clear separation of the positive population was observed.
Used the BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit
Bright staining, and low amounts of antibody needed.
Very expensive.
This clone with the pe-conjugation is performing as it should. Might be worth to look for other vendor options with a better price.