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Zombie Aqua™ Fixable Viability Kit
423101
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I used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.
Flow cytometry
THP-1 cells
10min
N/A
Zombie Viability dyes allowed me examine dead cell frequency in tissue culture samples and exclude them from further analysis. This dye worked well with when used prior to formaldehyde fixation. Although extending the incubation beyond 10 min led to increased nonspecific pick up of dye. Titration was also useful to determine optimum signal to noise ratio.
Reliable staining.
Titration is needed and provide with max incubation time.
A very reliable live dead dye to use before fixation of samples.