DAPI Staining for Confocal Microscopy

Università degli Studi Milano-Bicocca
Industrial Biotechnologies
Graduate Student

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Company:

Invitrogen™

Product Name:

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

Catalog Number:

D1306

We are studying the therapeutic potential of lipid nanoparticles loaded with drugs as a new system of drug delivery in osteosarcoma. Different tests are being performed such as cytotoxicity tests and uptake tests in a 2D model culture of murine osteosarcoma cells.

Experimental Design and Results Summary

Application

DAPI staining of murine cells for confocal microscopy

Starting Material

Murine osteosarcoma cells

Tips

Our lab used a concentration of 5mg/ml

Results Summary

The DAPI staining ensures robust and specific nuclear staining and allowed us to obtain really good images at the microscope.

DOI or PMID #

N/A

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Summary

The Good

High quality signal and easy to use.

The Bad

Really bright, pay attention to the amount used.

The Bottom Line

Its consistent performance, compatibility with diverse experimental conditions allowed us to get high quality images. Recommended!

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