Strong anti-mouse MHCII (I-A/I-E) BV395 antibody from BD BioScience

November 06, 2023

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD OptiBuild™ BUV395 Rat Anti-Mouse I-A/I-E

Catalog Number:

743876

Clone Number:

2G9

When used for flow cytometry on iMC38 tumor-infiltrating immune cells, there will be some background, but the staining on positive population is very strong

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor-infiltrating immune cells from MC38 subcutaneous tumor

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

Mouse tumor-infiltrating immune cells from MC38 subcutaneous tumors stained with this antibody show a very strong staining for MHCII

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very strong staining

The Bad

Some background is present

The Bottom Line

Overall, staining for this antibody is very strong. Background is present and can be decreased with performing antibody titration.

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