Study on the EGF - ERK Activity With Animal-Free Recombinant Human EGF From Pacific Coast Biologics

Molecular, Cellular, and Developmental Biology
UC Santa Barbara

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Company:

Pacific Coast Biologics

Product Name:

Animal Free Recombinant Human EGF

Catalog Number:

PC-AF-H-EGF

Clone Number:

N/A

We study the influence of numerous growth-promoting factors on ERK activity and how dynamic ERK activity shapes cell fate.

Experimental Design and Results Summary

Applications

Sample

Primary Incubation

Blocking Agent

Secondary Incubation

Tertiary Incubation

Detection

Results Summary

The image attached shows phosphorylated ERK in HEK293T cells after 10 hours of serum depletion (left) and following a 15-minute incubation in EGF (100ng/mL) (right).

DOI or PMID #

Additional Notes

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Summary

The Good

Pacific Coast Biologics Human EGF worked great as an ERK pathway activator.

The Bad

None

The Bottom Line

Our research is centered around the design and application of synthetic ligands to better understand receptor-level mechanisms of signal activation and demonstrate their potential for tissue engineering. We use natural ligands as a benchmark for standard pathway activity and to determine the potency of our synthetic ligands. 

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