L-Glutamine From Thermo Fisher is Ideal to Culture HL-1 Cells

Medical University of Graz
Biophysics
Postdoctoral Researcher

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Company:

Thermo Fisher Scientific

Product Name:

L-Glutamine (200 mM)

Catalog Number:

25030149

L-glutamine is diluted to 2 mM in Claycomb medium with 10% FBS, 1 % penicillin/streptomycin, and 0.1 mM noradrenaline and used for the culture of HL-1 cells.

Experimental Design and Results Summary

Application

Culture of cell lines.

Starting Material

Cell line, culture medium

Tips

Culture medium is changed every alternate day since L-glutamine degrades in culture medium at 37 degrees.

Results Summary

HL-1 cells attach to the culture flask preheated with fibronectin/gelatin and grow in the Claycomb culture medium containing 2 mM L-glutamine.

DOI or PMID #

N/A

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Summary

The Good

Easy to dilute and use in culture medium.

The Bad

Regular culture medium change is necessary to minimize L-glutamine degradation.

The Bottom Line

L-glutamine is suitable for culture of HL-1 cells.

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