Good Buffer From Stemcell Technologies for Isolating Mononuclear Cells

UC Davis
Health Division of Gastroenterology

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Company:

STEMCELL TECHNOLOGIES

Product Name:

Lymphoprep™

Catalog Number:

07801

We study circulating metabolites that regulate organismal physiology. We recently identified a few metabolites that are selectively induced upon infection in mice. We used this buffer to isolate monocytes from peripheral blood to test the effects of metabolites on monocytes in vitro.

Experimental Design and Results Summary

Application

Isolation of monocytes from blood

Starting Material

Tips

Keep in 4 degrees C.

Results Summary

We were able to isolate decent amount of monocytes from peripheral blood of human subjects using this ket and determined the pro-inflammatory effects of two metabolites.

DOI or PMID #

N/A

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Summary

The Good

Consistent quality.

The Bad

N/A

The Bottom Line

This is great buffer for isolating monocytes from peripheral blood of humans. Have not tried in mouse blood.

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