Efficient Recombinant Human IFN-Gamma from BioLegend

April 03, 2023

Dana-Farber Cancer Institute
CIV
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

Recombinant Human IFN-γ (carrier-free)

Catalog Number:

570202

This is a recombinant Human IFN-Y, which I used for activation of human monocyte cell line. THP-1 monocytes are differentiated into macrophages by 24 h incubation with 150 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139) followed by 24 h incubation in RPMI medium. Macrophages were polarized in M1 macrophages by incubation with 20 ng/ml of IFN-Y.

Experimental Design and Results Summary

Application

Differentiation of THP-1 cells

Starting Material

N/A

Tips

Regarding differentiation and activation of monocytes and macrophages, do not exceed the amount that is recommended by protocol. It causes over activation.

Results Summary

Adding IFNg led to activation of THP-1 cells and I used them for cytotoxicity assay. After differentiation of THP-1 cells, they will attach to the plate.

DOI or PMID #

N/A

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Summary

The Good

Affordable and efficient.

The Bad

N/A

The Bottom Line

In general, it could be used for different types of experiments, not only differentiation of cells, but also several other purposes. It can be used in cell culture, as an ELISA Standard, and as a Western Blot Control.

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