NK Expansion with IL-2

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Human IL-2 IS, premium grade 10 μg

Catalog Number:

130-097-744

I use Recombinant human IL-2 Improved Sequence (IS) at 500 UI/mL to expand and activate NK cells. It works very well.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

N/A

Tips

NK cell expansion varies by donor.

Results Summary

NK cells expanded with this IL-2 are highly actived, with a good viability and a functional profile. To perform cytotoxic assays, I usually do an overnight incubation without IL-2 so the cells do not massively degranulate in a non specific way.

DOI or PMID #

N/A

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Summary

The Good

Good quality.

The Bad

Expansive

The Bottom Line

Good quality IL-2, but quite expensive at the end when taken account the amount required for NK cells expansion.

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