tracrRNA for RNP Complex by IDT

January 03, 2023

Overall

Quality of Results

Ease-of-Optimization

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Company:

Integrated DNA Technology

Product Name:

Alt-R® CRISPR-Cas9 tracrRNA

Catalog Number:

1072533

I am performing CRISPR-Cas9 experiments on B cells and HEK to edit genes making membrane proteins. To do so, I am using a protocol for electroporation of RNP complex (crRNA+tracrRNA+Cas9).

Experimental Design and Results Summary

Application

Genetic edition

Starting Material

N/A

Tips

Use this in equimolar conditions with crRNA

Results Summary

This product enables us to perform efficiently gene knock-out. As shown on this picture, we can get 75% of knock-out cells using IDT tracrRNA.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reliable

The Bad

Expensive

The Bottom Line

This product is a must-have for CRISPR-Cas9 experiment as it is very easy to use, it has also a very good efficiency. Protocol given to use this reagent is also very easy to follow. I will continue to use this for my next experiments.

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