BD Biosciences
FITC Mouse Anti-Human IgM
555782
G20-127
Info: Supplier Page
Browse Similar Products: Mouse Anti-Human Secondary Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining igM in B cells (CD19+CD20+) in isolated PBMCs from peripheral blood of a healthy donor (HD).
Flow Cytometry
Heathy Donor PBMCs
anti-IgM FITC 1/50, 15', 4°C
Fc block
NA
Flow cytometer
We could stain IgM in B cells, and not in CD4 T cells.
It works and is specific
The staining is not bright
It is an option to stain human IgM