Retinoid Reporter Plasmid Working Well with Lentiviral Transduction

January 15, 2020

Princeton
Molecular Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

SystemBio

Product Name:

pGreenFire-RARE reporter plasmid

Catalog Number:

TR037PA-1

This was stably introduced into multiple cancer cell lines to check receptivity to retinoid signaling. Showed moderate signal in a small proportion of cells in distinct cell lines.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

MDA-468 cells

Tips

Using both retinoic acid and retinal yields signal

Results Summary

Results showed that a minority of MDA-MB-468 cells were active when this reporter was stably introduced as a lentiviral transductant and cells were treated with 10 mM retinal or retinoic acid.

DOI or PMID #

N/A

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Summary

The Good

No leakiness

The Bad

Minimal separation between positive and negative populations by flow

The Bottom Line

The is the only fluorescent reporter we could find to detect retinoid signaling, particularly as a lentiviral construct. Recommended more than any found at addgene.

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