Excellent Anti-Mouse IgG Antibody for Western Blot

January 21, 2020

Cell Biology
UC Berkeley
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Anti-mouse IgG, HRP-linked Antibody

Catalog Number:

7076S

Clone Number:

N/A

We use this anti-mouse as a secondary to our western blots, specifically a primary anti-mouse antibody. The detection works great for western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine T cells and muscle cells

Primary Incubation

1:1500 in 4% BSA

Blocking Agent

Nonfat dry milk

Secondary Incubation

1:5000 mouse antibody

Tertiary Incubation

N/A

Detection

ECL

Results Summary

The mouse antibody works very well and at 1:5000 dilution, it works if you will look at a different protein the day after. It isn't too bright that the membrane gets bleached therefor it is good for multi-day western blots.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great signal and excellent consistency

The Bad

None

The Bottom Line

This anti-mouse hrp will detect any mouse primary antibody that you have added.

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