Anti-IgM APC-Fire750 that Is Not a Much Better Alternative to Anti-IgM APC-Cy7

January 06, 2020

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Fire 750 anti-mouse IgM Antibody

Catalog Number:

406538

Clone Number:

RMM-1

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, I aimed at finding a brighter alternative to anti-IgM APC-Cy7.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 splenocytes

Primary Incubation

Surface staining with anti-IgM APC-Fire750, 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

I could stain IgM on the surface of B cells (B220+) but even at 1/100 dilution, the staining is not particularly bright.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It works and is specific

The Bad

Is not particular bright

The Bottom Line

It can be used

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