NEB Biolab Inc.
NEB® 10-beta Electrocompetent E. coli
C3020K
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We transformed 1ul of ligation mix into NEB® 10-beta Electrocompetent E. coli, ligated DNA mix with electrocompetent cell and directly put in electro cuvette and provide pulse with electroporator and recovered with 1 ml SOC medium provided with the kit and incubated for 1 hour at 37c. Plated 10 ul of SOC medium on to LB with antibiotic plates and incubated O/N at 37c.
Plasmid/ligated DNA transformation
Plasmid/Ligated DNA
Best for plasmid transformation size >10kb
Many positive colonies were identified after screening by using colony PCR.
N/A
Transformation efficient
Price
Excellent product to do a plasmid transformation of size >10kb.