BioLegend
Armenian hamster IgG PerCP/Cy5.5 isotype control
400931
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This antibody was used as an isotype control to account for non-specific background staining in mouse splenocytes. It was chosen because it was the recommended isotype control for an antibody in a phenotyping panel, and BioLegend had a competitive price.
Flow Cytometry
mouse splenocytes
1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
Isotype staining was minimal (filled histogram) as compared to unstained cells (dotted line).
Works well. Reproducible results. Low price.
Will continue to use this isotype control.