Biolegend
Anti-mouse FoxP3 AF647
126407
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: FOXP3 Flow Cytometry Antibodies
Our lab studies adoptive transfer of TCR gene modified T cells as immunotherapy for melanoma. We are examining mechanisms that alter the tumor microenvironment to induce a more inflammatory signature. We have looked at T cell infiltration into the tumor microenvironment and have identified increased frequency of T regulatory cells in untreated tumors compared to tumors treated with our transduced T cells.
Flow Cytometry
B16/A2 tumors excised from mice
2ul for 1 million cells for 30 minutes
None, examining T cells
None
Flow cytometry
I was able to detect differences in the frequency of T regulatory cells in the tumor microenvironment between treated and untreated tumors
N/A
Distinct populations were visible
The permeabilization steps are more time consuming, but clearly necessary for nuclear permeabilization
This is a good product to detect mouse T regulatory cells.