KAPA Biosystems
KAPA Library Quantification Complete Kit for Illumina
KK4824
Info: View Product View Product Specs
I have used KAPA Library quantification Kit for Illumina to quantify my DNA sequencing libraries. The kit is based on SYBR green based detection of DNA libraries and performs well with my samples in standard protocols as mentioned in the instruction manual. I have used this qPCR master mix in Agilent's Mx3500P suit and got consistent results on library quantification which eventually gave my optimal cluster amplifications in Miseq.
Quantification of DNA libraries for Illumina Miseq
Bacterial DNA libraries prepared with NEBNext Ultra DNA library prep kit.
Protocol is pretty simple and followed the instructions that come with the kit manual. First, prepare DNA standards which come with the kit and place them in 96 well microplates (each in 20 microliter reaction). Then prepare series of 10 fold dilutions on your DNA libraries and place them in the same 96 well microplate in 20 microliter reaction according to kit's instructions. qPCR cycling conditions and data analysis were performed as per instructions of the kit manual.
Do not use the kit if it is expired (as it says on the kit label)
Got consistent linear standard curve generation for DNA standards. My libraries were in the range of 50 nM concentration after qPCR quantification using KAPA quant kit.
None
Kit protocol is simple to perform. No complications.
Highly recommended for quantification of DNA libraries intended for use in Illumina sequencing platform.
Product Name: KAPA Library Quantification KitSupplier Page