Excellent PE Isotype Mouse IgG2b

Tisch Cancer Institute
Icahn School of Medicine at Mount Sinai
Post-Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE Mouse IgG2b, κ Isotype Ctrl Antibody

Catalog Number:

400314

This antibody was used as an isotype control in intracellular flow cytometry and compared to anti-HMGB1-PE to determine the specifity of the HMGB-1 antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine A20 lymphoma cell line

Primary Incubation

Recommended concentration, 20min, 4C

Blocking Agent

Blocking Buffer containing sera (rat, rabbit, human, goat)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (BD) LSRFortessa

Results Summary

Excellent isotype control antibody for flow cytometry. Murine A20 cells were fixed, permeabilied and stained with isotype or a PE antibody specific for HMGB1. Isotype shows low unspecific staining on usually very sticky tumor cells.

Additional Notes

None

Image Gallery

Summary

The Good

Excellent control

The Bad

None

The Bottom Line

Excellent istoype control antibody

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