Miltenyi Biotec
QuadroMacs Seperation Unit
130-090-976
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I am conducting research on Parkinson’s disease, looking specifically at how effector T cells are able to modulate and exacerbate the neurodegeneration seen in the substantia nigra of Parkinson’s patients. The Quadromacs Seperation unit is used to seperate CD4+ T cells from lymph nodes and spleens of immunized mice.
Cell culture
Splenocytes
Single cell suspensions made from spleens and lymph nodes of mice are incubated with antibody and then placed in a column that is attached to the quadromacs separation unit. Through negative selection, CD4+ T cells are run through the column and displaced into the elute that comes out of the column.
Be careful when transporting the column, it is polarized and will stick to metal surfaces very easily.
CD4+ T cells are negatively selected for on the column attached to the Quadromacs separation unit with a 85-90% purity.
None
The cells are negatively selected for with ~90% purity
Unit can be hard to handle
The unit is a great choice for negatively selecting cells.