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ELISA Max Standard Set Mouse IgE
432401
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I aimed to detect IgE levels in serum from transgenic mouse models. Circulating IgE is not very abundant, which makes this one of the trickiest Ig isotypes to do ELISA on. This kit performed admirably in sensitivity and consistency across replicates.
ELISA
Mouse serum
As per kit instructions.
Fairly straightforward
The results were fairly consistent over three biological replicates, as expected. This bodes well for technical reproducibility.
None
Consistent and sensitive
Trickiest part is ensuring that the assay is being run within the linear range of detection.
I would use again in future.
Product Name: Mouse IgE ELISA MAX™ StandardSupplier Page