Novus Biologicals
GPR120 Antibody
NBP1-00858
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Our goal in using this antibody was to look at the expression of mouse GPR120 in bone marrow. There are multiple mouse GPR120 antibodies available. We chose the Novus antibody because the company is known for their high quality of products.
Western blotting
C57BL6 bone marrow
GPCR GPR120 antibody, 1:1000 dilution in 5% bovine serum albumin (BSA) in phosphate-buffered saline (PBST).
5% milk in PBST
HRP-conjugated goat anti-rabbit antibody
N/A
Pierce ECL Western Blotting Substrate (from Pierce/Thermo Scientific, cat #32209), used according to manufacturer’s instructions.
Antibody detects the target band of GPR120 (42kD), although it has some non-specificity.
None.
Can detect GPR120 in bone marrow samples.
Specificity.
A good antibody for detection of GPCR120 in bone marrow.