Parallel Analysis of The Proteome, Histone PTMs, and RNA Modifications from Frozen and FFPE Tissue by MS
Joanna Lempiäinen, Ph.D.
Friday, June 9, 2023
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Chapters
Introduction
Malignant Peripheral Nerve Sheath Tumor (MPNST)
Loss of PRC2 Predicts Poor Survival
How Does PRC2 Loss Promote Sarcoma Progression?
Current Project: Role of SUZ12 and EED in MPNST
Covaris Sonication Combined with S-trap is Most Efficient for FFPE Protein Extraction
Current Project: Role of SUZ12 and EED in MPNST
FFPE and Frozen Tissue Workflows
Settings Used for Proteome Analysis
Sonication Optimization
FFPE Proteomes Correlate with Frozen Proteomes
More Than 100 Histone PTMs/Combinations Identified in One Sample
Workflows Successfully Identify RNA Modifications from FFPE and Frozen Samples
Covaris Sonication for ChIP-seq
Summary
Acknowledgements
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