The use of recombinant epitope tags has become a staple tool in identifying, characterizing and purifying proteins for which no specific antibody is readily available.
Instead, the inherent properties of the tags allow for purification and detection by readily binding to corresponding antibodies or certain molecules.
They typically are composed of short peptides or small proteins, and ideally should not interfere with the structure and function of the protein of interest.
That is not always the case, however, and fusion protein sequences often include a cleavage site for removal of the tag.
When paired with antibodies specific to the epitope sequence, they are useful methods in many applications such as immunoprecipitation, immunohistochemistry, immunocytochemistry, immunofluorescence, blotting, ELISA, and flow cytometry.
To aid in your research, browse our diverse catalog of epitope tag antibodies from different suppliers.
Some frequently used epitope tags:
DYKDDDDK tag
- the octapeptide DYKDDDDK tag, which consists of aspartic acid, tyrosine, and lysine residues, is less likely to denature proteins due to its hydrophilic nature.
GST tag
- Glutathione S-transferase (GST) is a large epitope tag comprising 220 amino acids in length and roughly 26 kDa in mass. It has a strong affinity for glutathione (GSH).
HA tag
- The HA tag is derived from the human influenza hemagglutinin and consists of the 9-amino acid sequence YPYDVPDYA.
His tag
- also known as 6xHis tag and polyhistidine tag, this epitope comprises six histidine residues (HHHHHH) that bind to metal ions, usually nickel, zinc and cobalt.
Myc tag
- this 10-amino acid polypeptide tag (EQKLISEEDL) is derived from C-terminus of the human c-Myc protein.
S tag
- derived from RNase A, S-tag contains the 15-amino acid sequence KETAAAKFERQHMDS.
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