Antibody Purification involves selective enrichment or specific isolation of antibodies from serum, ascites fluid, or cell culture supernatant. Purification is required to obtain antibody probes that can be used effectively in multiple types of detection methods. There are a variety of purification methods, ranging from non-specific to highly specific. The methods are classified under physiochemical fractionation, class-specific affinity, or antigen- specific affinity, in increasing order of specificity. When choosing the appropriate purification method keep in mind both the antibody of interest as well as the future downstream analysis.
Tips on how to design and evaluate efficacy as well as troubleshooting...
Some methods are quantitative, label-free, and can track in real-time.
I work with in-vitro protein phase separation assays which require high-quality ...
The WNT pathway is essential for embryonic development and adult tissue ...
Esco Labculture A2 (LA2) G4 series BSCs are full-sized, high performance, ...
Affinity is an important parameter for evaluating molecular interactions and ...