Description
CST's PathScan® Acetyl-Histone H3 (Lys9) Sandwich ELISA Kit #7121 detects endogenous levels of histone H3 when acetylated at Lys9. As shown in Figure 1 using the Acetyl-Histone H3 (Lys9) Sandwich ELISA Kit #7121, a high level of acetylation at Lys9 on histone H3 is detected in COS cells when treated with TSA. The level of total histone H3 (modified and unmodified) remains unchanged as shown by Western analysis (Figure 1). Similar results are obtained when NIH/3T3 and Jurkat cells are treated with TSA (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species