Fig 1: The critical molecule and signaling pathway related to the angiogenic function of Tagln2. (A, B) Tagln2-derived expression of angiogenesis factors and adhesion molecular in ECs. Primary HUVECs infected with lentiviral expression vector directing the expression of TAGLN2 or with mock vectors were prepared. The RayBiotech Human Angiogenesis Antibody Array 1000 and Adhesion Molecule Array Q1 (Cat#QAH-ANG-2 and QAH-CAM-1) were purchased from RayBiotech Inc. (Norcross, GA, USA). Blots were scanned with an InnoScan 300 Microarray Scanner and analyzed using ImageJ software. Proteins with band densities exhibiting a fold increase of greater than 1.5 or a fold decrease of greater than 0.67 were defined as differentially expressed proteins. The relationship between 26 differentially expressed proteins from the angiogenesis antibody array and 47 upregulated DEGs identified by RNA-Seq were analyzed and deeply mined using the STRING website (https://string-db.org/). A protein-protein interaction network was constructed (C), and the corresponding Top 10 enriched biological processes and KEGG pathways (D) were shown. **P < 0.01, ***P < 0.001.