Fig 1: DSS treatment did not cause systemic inflammation in male rats of either genotype.Serum cytokines were assayed using a rat cytokine antibody array (AAR-CYT-1; RayBiotech, Norcross, GA). A grid was drawn on one blot for orientation, and positive (green) and negative (red) controls are indicated with boxes. The positive and negative controls worked as expected, validating the assay. The layout of the blots is shown in the table below (as provided by the manufacturer). Only two cytokines were positive MCP-1 (yellow boxes) and TIMP-1 (purple boxes), but levels were not noticeably different between genotypes or treatment groups.
Supplier Page from RayBiotech for Rat Cytokine Array C1