Anti-biotin, HRP-linked Antibody 7075S from Cell Signaling Technology

Product Specs
ItemAnti-biotin, HRP-linked Antibody
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7075S
Quantity1 ml
HostGoat
Conjugate/Tagbiotin
Research AreaRelated Products
TargetBiotin (for Secondary Abs)
ApplicationsW
ReactivityAll

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) BioE3 identifies specific substrates of ubiquitin E3 ligasesNature CommunicationsNovember 23, 2023Orhi Barroso-Gomila, Laura Merino-Cacho, Veronica Muratore, Coralia Perez, Vincenzo Taibi, Elena Maspero, Mikel Azkargorta, Ibon Iloro, Fredrik Trulsson, Alfred C. O. Vertegaal, et al.at RT. Primary antibodies used as follows: Cell Signaling Technology: anti-biotin-HRP (1/1000; Cat#7075 S), anti-alpha-Actinin (1/5000; Cat#6487S), anti-PCM1 (1/1000; Cat#5213S); SinoBiological: anti-BirA

(2) Prolyl hydroxylase 2 inhibits glycolytic activity in colorectal cancer via the NF‑κB signaling pathwayInternational Journal of OncologyNovember 15, 2023Lisha Xiang, Hao Wei, Wentao Ye, Shuang Wu, Ganfeng XieInc.), -mouse (1:5,000; cat. no. 7076; Cell Signaling Technology, Inc.), or -goat (1:5,000; cat. no. 7075; Cell Signaling Technology, Inc.) secondary antibodies were used for incubation for 1 h at room temperature. Electrophoretic mobility shift assay

(3) Garcinia mangostana L. Pericarp Extract and Its Active Compound α-Mangostin as Potential Inhibitors of Immune Checkpoint Programmed Death Ligand-1MoleculesOctober 9, 2023Sandar Naing, Nichawadee Sandech, Arnatchai Maiuthed, Sumet Chongruchiroj, Jaturong Pratuangdejkul, Pattamapan Lomaratperoxidase (HRP)-conjugated anti-rabbit IgG (#7074, Cell Signaling Technology, MA, USA) and anti-biotin (#7075, Cell Signaling Technology, MA, USA) at 1:2000 dilution. The protein bands were exposed using enhanced

(4) Click chemistry-based thiol redox proteomics reveals significant cysteine reduction induced by chronic ethanol consumptionRedox BiologyAugust 1, 2023Peter S. Harris, Courtney D. McGinnis, Cole R. Michel, John O. Marentette, Richard Reisdorph, James R. Roede, Kristofer S. Fritzsignal from specific antibodies. The anti-biotin antibody was purchased from Cell Signaling Technology (7075) and was used at 1:1000 dilution. Blots were visualized using a Bio-Rad ChemiDoc MP Imaging System and

(5) GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of SnailCell death & diseaseJune 28, 2023Zou X, Ma L, Zhang Y, Zhang Q, Xu C, Zhang D, Chu Y, Zhang J, Li M, Zhang H, et al.mouse anti-Snail (sc-271977, Santa Cruz), rabbit anti-p66β (ab76925, Abcam), anti-biotin, HRP-linked (7075, Cell Signaling Technology), and rabbit anti-IgG (2729, Cell Signaling Technology), mouse anti-α-tubulin

(6) GATS tag system is compatible with biotin labelling methods for protein analysisScientific ReportsJune 23, 2023Kohdai Yamada, Fumiya Soga, Soh Tokunaga, Hikaru Nagaoka, Tatsuhiko Ozawa, Hiroyuki Kishi, Eizo Takashima, Tatsuya Sawasakilaboratory), α-tubulin (MBL, PM054-7, 1:5000), GST (M071-7, 1:5000), and biotin (Cell Signaling Technology, #7075, 1:1000). The following primary antibodies were used in this study: IκBα (CST L35A5, 1:1000), RelA (CST

(7) RBBP4 is an epigenetic barrier for the induced transition of pluripotent stem cells into totipotent 2C-like cellsNucleic Acids ResearchJune 23, 2023Wangfang Ping, Yingliang Sheng, Gongcheng Hu, Hongxin Zhong, Yaoyi Li, YanJiang Liu, Wei Luo, Chenghong Yan, Yulin Wen, Xinxiu Wang, et al.biotinylation of RBBP4 and RBBP7 was detected with anti-Biotin antibody (Cell Signaling Technology, 7075). Trophoblast derivation from mESCs Cell culture plates were pre-coated with 0.3% gelatin. Cdx2-tdTomato

(8) Identification of a new gibberellin receptor agonist, diphegaractin, by a cell-free chemical screening systemCommunications BiologyMay 9, 2023Akira Nozawa, Ryoko Miyazaki, Yoshinao Aoki, Reina Hirose, Ryosuke Hori, Chihiro Muramatsu, Yukinori Shigematsu, Keiichirou Nemoto, Yoshinori Hasegawa, Keiko Fujita, et al.with horse radish peroxidase-conjugated anti-biotin antibody (Cell Signaling Technology, Danvers, MA, #7075, 1:5000) or anti-FLAG antibody (Sigma Aldrich Japan, A8592, 1:5000). The synthesised proteins were detected

(9) Mitochondrial calcium uniporter activates TFEB-driven autophagy to promote migration of breast cancer cellsIran J Basic Med SciApril 4, 2023Lin Yuan, Qimou Lin, Fei Shen, Yong Li, Junda Li, Bo Xucat.7074, Beverly, USA) and anti-biotin, HRP-linked antibody (1:2000; Cell Signaling Technology, cat.7075, Beverly, USA) for 1 hr at room temperature. The membranes were then visualized using the enhanced chemiluminescence

(10) Diverse yeast antiviral systems prevent lethal pathogenesis caused by the L-A mycovirusProceedings of the National Academy of SciencesMarch 14, 2023Sabrina Chau, Jie Gao, Annette J. Diao, Shi Bo Cao, Amirahmad Azhieh, Alan R. Davidson, Marc D. Meneghiniat 4°C overnight and probed with 1:3,000 horseradish peroxidase (HRP)-conjugated horse anti-mouse (7075; Cell Signaling Technology) or goat anti-rabbit (7074; Cell Signaling Technology) secondary antibody.

(11) P-Rex1 is a novel substrate of the E3 ubiquitin ligase Malin associated with Lafora diseaseNeurobiology of DiseaseFebruary 1, 2023L. Kumarasinghe, M.A. Garcia-Gimeno, J. Ramirez, U. Mayor, J.L. Zugaza, P. Sanzrabbit anti-GLUT1 (PA1–46152. Invitrogen. Madrid, Spain), goat anti-biotin-HRP-conjugated antibody (#7075. Cell Signaling Technology, Barcelona, Spain), and mouse anti-Na+/K+-ATPase (ab7671. Abcam. Madrid,

(12) CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysisScientific ReportsDecember 1, 2022Shusei Sugiyama, Kohdai Yamada, Miwako Denda, Satoshi Yamanaka, Satoshi Ozawa, Ryo Morishita, Tatsuya SawasakiAGIA (produced in our laboratory)41, α-tubulin (MBL, PM054-7), and biotin (Cell Signaling Technology, #7075). To detect the proteins spotted on the array, anti-DDDDK-tag mAb-Alexa Fluor 647 (MBL, M185-A64) was

(13) Processes in DNA-damage response from a whole-cell multi-omics perspectiveiScienceNovember 18, 2022James C. Pino, Alexander L.R. Lubbock, Leonard A. Harris, Danielle B. Gutierrez, Melissa A. Farrow, Nicole Muszynski, Tina Tsui, Stacy D. Sherrod, Jeremy L. Norris, John A. McLean, et al.Signaling), PLK1 (ab17056, Abcam), or phosphorylated PLK1 (ab133442, Abcam). Binding of an anti-mouse (7075, Cell Signaling) or an anti-rabbit (7074, Cell Signaling) HRP-conjugated secondary antibody was detected

(14) Caveolae couple mechanical stress to integrin recycling and activationeLifeOctober 20, 2022Lolo FN, Pavón DM, Grande A, Elósegui Artola A, Segatori VI, Sánchez S, Trepat X, Roca-Cusachs P, Del Pozo MA.Alexa Fluor–488 phalloidin (Thermo Fisher Scientific); HRP-linked anti-biotin from Cell Signaling (#7075); and Alexa Fluor–647 phalloidin (Thermo Fisher Scientific). EZ-Link SulfoNHS-SS-biotin was from Thermo

(15) Aberrant Cholesterol Metabolism and Wnt/β-Catenin Signaling Coalesce via Frizzled5 in Supporting Cancer GrowthAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2022Zheng S, Lin J, Pang Z, Zhang H, Wang Y, Ma L, Zhang H, Zhang X, Chen M, Zhang X, et al.Ki‐67 (8D5) Mouse mAb,Cell Signaling Technology,#9499 "Anti‐biotin, HRP‐linked Antibody",Cell Signaling Technology,#7075 Anti‐Frizzled 5 Rb Polyclonal Antibody,Sigma‐Aldrich,06‐756 Wnt3a (C64F2) Rabbit mAb,Cell Signaling Technology

(16) In vitro sepsis induces Nociceptin/Orphanin FQ receptor (NOP) expression in primary human vascular endothelial but not smooth muscle cellsPLOS ONESeptember 15, 2022Mark F. Bird, Barbara Gallacher-Horley, John McDonald, David G. McVey, Fatin Al-Janabi, Remo Guerrini, Girolamo Calo, Shu Ye, Jonathan P. Thompson, David G. LambertHRP-conjugated secondary antibodies (1hr room temperature; 1:1000 dilution 5% milk/TBS-T solution; #7075, Cell Signalling Technology). Chemiluminescence detection, using the ChemiDoc™ MP imaging system (Bio-Rad,

(17) Non-Apoptotic Caspase-3 Activation Mediates Early Synaptic Dysfunction of Indirect Pathway Neurons in the Parkinsonian StriatumInternational Journal of Molecular SciencesMay 13, 2022Tim Fieblinger, Chang Li, Elena Espa, M. Angela Cenciwere incubated with HRP-linked secondary antibodies (anti-biotin HRP-linked antibody Cell Signaling, #7075; anti-rabbit-IgG HRP-linked antibody, Cell Signaling, #7074) and bands were visualized by chemiluminescence

(18) Juvenile Hormone Membrane Signaling Enhances its Intracellular Signaling Through Phosphorylation of Met and Hsp83Frontiers in PhysiologyApril 27, 2022Yue Gao, Nan Chen, Xiangle Zhang, Emma Y. Li, Wei Luo, Jie Zhang, Wenqiang Zhang, Sheng Li, Jian Wang, Suning Liuby chemiluminescence. The primary antibodies used in this study were HRP-linked goat anti-biotin (#7075, Cell Signaling Technology, CST), mouse anti-FLAG (#F1804, Sigma-Aldrich), rabbit anti-V5 (#ab9116,

(19) Integrin mediates cell entry of the SARS-CoV-2 virus independent of cellular receptor ACE2The Journal of biological chemistryMarch 1, 2022Liu J, Lu F, Chen Y, Plow E, Qin J.antibody (Novagen #70796), and biotinylated proteins were detected by biotin antibody (Cell signaling #7075). SPR experiments SPR experiments were carried out on the BIAcore T200 (Cytiva, former GE Healthcare)

(20) Differential maturation and chaperone dependence of the paralogous protein kinases DYRK1A and DYRK1BScientific reportsFebruary 14, 2022Papenfuss M, Lützow S, Wilms G, Babendreyer A, Flaßhoff M, Kunick C, Becker W.polyclonal anti-mouse IgG (H&L, #610-703-124, Rockland, Gilbertsville, PA, USA), goat anti-biotin IgG (#7075, Cell Signaling Technology, Danvers, MA, USA). Plasmids The NEBuilder Assembly Tool v2.2.7 and the

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(1)

5/27/2020HRP-Conjugated Anti-Biotin Antibody Working Well for WB Wei XuI used this antibody to detected biotin signals by western blot and it worked well.

Anti-biotin, HRP-linked Antibody from Cell Signaling Technology

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Cell Signaling Technology

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Anti-biotin, HRP-linked Antibody from Cell Signaling Technology

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