Fig 2: Treatment with QNZ reduces renal tissue damages and IgG and complement 3 depositions in the kidney tissues of mice. Following treatment with QNZ or FK-506 for 8 weeks, the mice were perfused and euthanized. Their kidney tissues were dissected and the paraffin-embedded kidney tissue sections were stained with H&E and PAS. In addition, the kidney tissues were embedded in OCT and frozen, and their crystal sections were stained with fluorescent antibodies against mouse IgG or C3 (red color), followed by nuclearly staining with DAPI. (A) H&E and PAS staining of kidney tissue sections. Scale bar: 20 μm. (B, C) Immunofluorescent analysis of IgG1 and C3 deposition in the kidney tissues. Scale bar: 20 μm. (D) Different groups of mice were scored for glomerular abnormalities on a scale from 0 to 4. (E) The per area (objective/total×100%) of inflammatory infiltration in H&E staining of kidney section. (F, G) Mean fluorescence intensity (MFI) of IgG and C3 staining. The data are representative images from each group of mice. The data are expressed as the mean ± SD. (n = 4–6 independent experiments/group) *p < 0.05, **p < 0.01.