Cell-free expression systems allow for the in-vitro synthesis of a protein using only the cellular machinery required for transcription and translation, enabling quick manufacture of small amounts of functional proteins without the need for transfection and cell culture. Traditional cell-free expression methods use crude cell extracts derived from rabbit reticulocytes, wheat germ, or Escherichia coli to drive protein translation. Other approaches use coupled translation and transcriptions systems using reconstituted ribosomes and RNA polymerase components purified from E. coli. The proteins that carry out transcription/translation are his-tagged, allowing for easy purification of translated proteins in their native state. The reaction is also more well-defined and void of nucleases and proteases that may be present in crude extracts. Cell-free expression systems can be used for the production of functional virions, expression of active mammalian proteins with post-translational modifications, generating protein arrays, and expression of mutant or truncated proteins. Visit the supplier page for more product information.
Your search returned 33 Cell-Free Expression Systems across 4 suppliers.
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New England Biolabs
- 100 reactions
- Proteomics & Glycomics
- 10 reactions
- Proteomics & Glycomics
- PURExpress Disulfide Bond Enhancer
- Proteomics & Glycomics
Promega
- Rabbit Reticulocyte Lysate Translation Systems are used in identification of mRNA species, characterization of their ...
- 30 reactions
- Inquire
- The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from ...
- 24 reactions
- Inquire
- The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from ...
- 8 reactions
- Inquire
Bioneer, Inc.
- AccuRapid Cell-Free Protein Expression Kit helps cell-free protein expression with E. coli extract in mini scale in 3-...
- 24 RXN
- Protein Expression
- AccuRapid Protein Synthesis Kit combines cell-free protein expression with E. coli extract and Ni-NTA magnetic bead-...
- 5 RXN
- Protein Expression and Purification, Protein Expression/...
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- ExiProgen EC Protein Synthesis Kit, used in ExiProgen instrument, can express and purify 1-16 different proteins ...
- 16 RXN
- Protein Expression and Purification, Protein Expression/...
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Creative BioMart
- 45 µL x 24 reactions
- Inquire
- 10 mL x 1 reaction
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- 1 mL x 5 reactions
- Inquire
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