Sequel System from Pacific Biosciences

Product Specs
ItemSequel System
CompanyPacific Biosciences
Price Supplier Page View Company Product Page
Catalog NumberSequel System
Read Length1 kb and up
Maximum Reads1 - 10 M
Maximum Output1 - 10 Gb
ApplicationsNext Gen Sequencing, Whole Genome Sequencing, De Novo Assembly, RNA-Seq, Targeted Sequencing, Amplicon Sequencing, Metagenomics
Run Time0.5 - 10 hours
Sample ThroughputCan process up to 16 SMRT cells serially per run
Data AnalysisUp to 365,000 reads, up to 7.6 Gb output
DimensionsWxDxH: 36.5 x 34 x 66 in / 92.7 x 86.4 x 167.6 cm
Quantity1 system

Pacific Biosciences

Phone: 650.521.8000

Website: http://www.pacificbiosciences.com/

(1) Improved chromosome-level genome assembly of Indian sandalwood (Santalum album)Sci DataDecember 21, 2023Xinhua Zhang, MingZhi Li, Zhan Bian, Xiaohong Chen, Yuan Li, Yuping Xiong, Lin Fang, Kunlin Wu, Songjun Zeng, Shuguang Jian, et al.as the 20 kb SMRTbell templates. PacBio long reads were sequenced for two SMRT cells on the PacBio Sequel System (Pacific Biosciences, CA, USA) at the BGI. Errors in the PacBio SMRT sequences were initially corrected

(2) HLA-B*46:01:01:01 and HLA-DRB1*09:01:02:01 are associated with anti-rHuEPO-induced pure red cell aplasiaSci RepDecember 20, 2023Thitima Benjachat Suttichet, Monpat Chamnanphon, Monnat Pongpanich, Sarun Chokyakorn, Pawinee Kupatawintu, Chalurmpon Srichomthong, Wanna Chetruengchai, Hathaichanok Chuntakaruk, Thanyada Rungrotmongkol, Pajaree Chariyavilaskul, et al.equimolar pooling of each sample's HLA Class I and Class II. The HLA libraries were sequenced in the PacBio Sequel System (Sequencing primer V3, movie time of 10 h per SMRT cell, Pacific Biosciences Inc., Menlo Park, CA, USA).

(3) Genomic attributes and characterization of novel exopolysaccharide-producing bacterium Halomonas piscis sp. nov. isolated from jeotgalFront MicrobiolDecember 14, 2023Bora Kim, Ah-In Yang, Hae-In Joe, Ki Hyun Kim, Hanna Choe, Sung-Hong Joe, Min Ok Jun, Na-Ri Shin(Macrogen, Republic of Korea). Genomic DNA was extracted from strain SG2L-4T and sequenced using the PacBio Sequel system and Illumina HiSeq sequencing platform. The PacBio reads were used for de novo assembly via Microbial

(4) Discovery and structure elucidation of glycosyl and 5-hydroxy migrastatins from dung beetle gut Kitasatospora sp.J Ind Microbiol BiotechnolDecember 13, 2023Ji Hyeon Im, Seoyoung Oh, Eun Seo Bae, Soohyun Um, Sang Kook Lee, Yeon Hee Ban, Dong-Chan Ohwas acquired from CJ Bioscience, Inc. (Seoul, Republic of Korea) (Yoon et al., 2017) using the PacBio Sequel system (Pacific Biosciences, USA) (Rhoads & Au, 2015). A hybrid de novo assembly was constructed using SMART

(5) Chromosome-level genome assembly and annotation of the Antarctica whitefin plunderfish Pogonophryne albipinnaSci DataDecember 12, 2023Euna Jo, Soyun Choi, Seung Jae Lee, Jinmu Kim, Eun Kyung Choi, Minjoo Cho, Jangyeon Kim, Sangdeok Chung, Jaebong Lee, Jeong-Hoon Kim, et al.SMRTbell library was then prepared as per the manufacturer’s protocol. Sequencing was conducted on a Sequel system (Pacific Biosciences, Menlo Park, CA, USA) using two SMRT cells 1 M v3 LR and Sequel sequencing chemistry

(6) Comparative genomics and genome-wide SNPs of endangered Eld’s deer provide breeder selection for inbreeding avoidanceScientific ReportsNovember 13, 2023Vichayanee Pumpitakkul, Wanna Chetruengchai, Chalurmpon Srichomthong, Chureerat Phokaew, Wirulda Pootakham, Chutima Sonthirod, Wanapinun Nawae, Sissades Tongsima, Pongsakorn Wangkumhang, Alisa Wilantho, et al.blood. For SED, long-read and short-read sequencing was conducted using the Pacific Biosciences (PacBio) Sequel system and Illumina NovaSeq 6000 platform, which produced 147.8 and 97.0 Gb of raw data, respectively. Hybrid

(7) Chromosome-level genome assembly of the deep-sea snail Phymorhynchus buccinoides provides insights into the adaptation to the cold seep habitatBMC GenomicsNovember 10, 2023Zhaoqun Liu, Yuting Huang, Hao Chen, Chang Liu, Minxiao Wang, Chao Bian, Lingling Wang, Linsheng SongUSA), one SMART bell library was constructed. The SMRT sequencing was performed on Pacific Bioscience Sequel System (Pacific Biosciences, CA, USA). Assemblies of genome and transcriptome All cleaned reads from short

(8) Genome sequence resource for “Candidatus Liberibacter asiaticus” strain GDCZ from a historical HLB endemic region in ChinaBMC Genomic DataNovember 4, 2023Yongqin Zheng, Yun Li, Pengbin Xu, Chaoji Liu, Jianchi Chen, Xiaoling Deng, Zheng Zhengstrain GDCZ contained a Type 2 phage and a CLasMV1 phage. Genome sequencing was performed by a PacBio Sequel system with 20-kb library insert size (Pacific Biosciences, Menlo Park, CA, U.S.A.) and an Illumina Hiseq Xten

(9) Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas speciesBiotechnology for Biofuels and BioproductsOctober 11, 2023Matthew Faulkner, Robin Hoeven, Paul P. Kelly, Yaqi Sun, Helen Park, Lu-Ning Liu, Helen S. Toogood, Nigel S. Scruttonbarcoded overhang adapter kit (Pacific Biosciences). Data were acquired using the Pacific Biosciences Sequel system. Following demultiplexing the genomic sequences were assembled using the Resequencing algorithm of SMRT

(10) Characterisation of Milk Microbiota from Subclinical Mastitis and Apparently Healthy Dairy Cattle in Free State Province, South AfricaVeterinary SciencesOctober 11, 2023N. G. Khasapane, Z. T. H. Khumalo, S. Kwenda, S. J. Nkhebenyane, O. Thekisoe(v 4.3.1) package [27], was used to analyse raw amplicon sequencing data generated using the PacBio Sequel System (Pacific Biosciences, Menlo Park, CA, USA). Briefly, raw amplicon data were initially processed using

(11) Microbiota Profiles of Hen Eggs from the Different Seasons and Different Sectors of Shanghai, ChinaMicroorganismsOctober 9, 2023Haiyan Gong, Yingqing Ma, Min Wang, Yumeng Gu, Ruipeng Deng, Bo Deng, Dongsheng Feng, Yiyi Han, Rongsheng Mi, Yan Huang, et al.sequencing libraries. The libraries were then treated with high-throughput sequencing (HTS) using a PacBio Sequel system (PacBio) [12], which resulted in data in bam format. These data were exported as CCS files via the SMRTlink

(12) Predicting Blomia tropicalis allergens using a multiomics approachClinical and Translational AllergyOctober 1, 2023Jan Hubert, Susanne Vrtala, Bruno Sopko, Scot E. Dowd, Qixin He, Pavel B. Klimov, Karel Harant, Pavel Talacko, Tomas Erbanusing 40% diluted AMPure PB Beads. Each library was then sequenced using a 10‐h movie time on a PacBio Sequel system (Pacific Biosciences). The SMRT Link Circular Consensus Sequencing workflow (SMRT Link v.9.0.0, CCS)

(13) Draft genome sequence of Limosilactobacillus reuteri, isolated from human breast milkMicrobiology Resource AnnouncementsSeptember 19, 2023Akanksha Tyagi, Su-Jung Yeon, Ramachandran Chelliah, Deog-Hwan Ohlibrary was built with the SMRTbell Express template preparation kit v2.0 (PacBio) with 3 µg of gDNA. The Sequel system (PacBio) was used to sequence the library, yielding 72,860 subreads, N50 10,519 bp, with an average

(14) Evidence supportive of a bacterial component in the etiology for Alzheimer’s disease and for a temporal-spatial development of a pathogenic microbiome in the brainFrontiers in Cellular and Infection MicrobiologySeptember 13, 2023Yves Moné, Joshua P. Earl, Jarosław E. Król, Azad Ahmed, Bhaswati Sen, Garth D. Ehrlich, Jeffrey R. Lapidesin equimolar concentration into multiplexed sets of 2 to 18 samples per pool. Pacific Biosciences Sequel System sequencing Sequencing libraries were constructed from each pool of barcoded amplicons using the SMRTbell

(15) High quality de novo genome assembly of the non-conventional yeast Kazachstania bulderi describes a potential low pH production host for biorefineriesCommunications BiologySeptember 7, 2023Laura N. Balarezo-Cisneros, Soukaina Timouma, Alistair Hanak, Andrew Currin, Fernando Valle, Daniela DelneriBiosciences). DNA libraries were sequenced using the SMRT Cell 1 M chips on the Pacific Biosciences Sequel system with 10 h data acquisition time. For Nanopore sequencing, 1 µg of DNA samples (not sheared) were prepared

(16) Transcriptome Responses to Different Environments in Intertidal Zones in the Peanut Worm Sipunculus nudusBiology (Basel)August 29, 2023Junwei Li, Jiufu Wen, Ruiping Hu, Surui Pei, Ting Li, Binbin Shan, Honghui Huang, Changbo ZhuAssembly Each library pool was sequenced independently on six SMRT cells of a Pacific Biosciences Sequel system generating a total of 21.72 Gbp of raw reads. Demultiplexing, stringent filtering, and quality control

(17) Transcriptomic analysis of Amaranthus retroflex resistant to PPO-inhibitory herbicidesPLoS OneAugust 24, 2023Yulian Guo, Yu Wang, Xiangyun Zang, Chan Luo, Chunyan Huang, Keqiang Cong, Xiaotong GuoCalifornia, Inc). PacBio data analysis The PacBio raw reads generated from SMRT Sequencing on the Sequel System were analyzed to obtain reads of insert (ROI), read classification, and read clusters using Quvier and

(18) Chromosome-level genome assembly of Korean native cattle and pangenome graph of 14 Bos taurus assembliesSci DataAugust 23, 2023Jisung Jang, Jaehoon Jung, Young Ho Lee, Sanghyun Lee, Myunggi Baik, Heebal KimIso-Seq, a total of 600 ng RNA from sirloin was used for full-length transcript sequencing with Pacbio Sequel system (Pacific Biosciences, CA, USA) according to the manufacturer’s instructions. The Iso-Seq library was

(19) In Vivo Microevolutionary Analysis of a Fatal Case of Rhinofacial and Disseminated Mycosis Due to Azole-Drug-Resistant Candida SpeciesJ Fungi (Basel)August 2, 2023Yuchen Wang, Xi Guo, Xinran Zhang, Ping Chen, Wenhui Wang, Shan Hu, Teng Ma, Xingchen Zhou, Dongming Li, Ying Yangwas prepared from sheared genomic DNA according to the library preparation workflow, and the PacBio Sequel system was used to conduct SMRT sequencing using C3 sequencing chemistry and P5 polymerase with 16 SMRT cells

(20) High-Quality Genome Assembly and Genome-Wide Association Study of Male Sterility Provide Resources for Flax ImprovementPlants (Basel)July 26, 2023Xiaoqing Zhao, Liuxi Yi, Yongchun Zuo, Fengyun Gao, Yuchen Cheng, Hui Zhang, Yu Zhou, Xiaoyun Jia, Shaofeng Su, Dejian Zhang, et al.with sequencing primers was performed. Finally, the SMRTbell library was sequenced using the PacBio Sequel System, also from Pacific Biosciences (Menlo Park, CA, USA). For the construction of short-read paired-end

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