12 mm Snapwell™ Insert with 0.4 µm Pore Polyester Membrane, Sterile from Corning Life Sciences

Product Specs
Item12 mm Snapwell™ Insert with 0.4 µm Pore Polyester Membrane, Sterile
CompanyCorning Life Sciences
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3801
Quantity6 / Pk
Brand/TrademarkCorning®
SterilitySterile
TypeInserts

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Corning Life Sciences

836 North Street

Building 300 Suite 3401

Tewksbury, MA 01876

United States

Phone: (978) 442-2200
Phone: (800) 492-1110

Email: clscustserv@corning.com

Company Profile

Website: www.corning.com/lifesciences

(1) Discovery of Fungus-Derived Nornidulin as a Novel TMEM16A Inhibitor: A Potential Therapy to Inhibit Mucus Secretion in AsthmaJournal of Experimental PharmacologyNovember 15, 2023Pawin Pongkorpsakol, Chantapol Yimnual, Wilasinee Satianrapapong, Nichakorn Worakajit, Suchada Kaewin, Praphatsorn Saetang, Vatcharin Rukachaisirikul, Chatchai MuanprasatCO2. For Ussing chamber experiments, Calu-3 cells were seeded onto Snapwell inserts (catalog number: 3801) (Costar, Cambridge, Massachusetts, USA) at a density of 5×105 cells/well and grown in a humidified

(2) Protospacer modification improves base editing of a canonical splice site variant and recovery of CFTR function in human airway epithelial cellsMolecular Therapy - Nucleic AcidsSeptember 12, 2023Anya T. Joynt, Erin W. Kavanagh, Gregory A. Newby, Shakela Mitchell, Alice C. Eastman, Kathleen C. Paul, Alyssa D. Bowling, Derek L. Osorio, Christian A. Merlo, Shivani U. Patel, et al.plated onto human collagen type IV (Sigma, no. C6745-1ML) coated snapwell filters (Costar, catalog no. 3801) in a 6-well plate seeded with irradiated mouse fibroblast cells (3T3). Two electroporation reactions

(3) Clinical Consequences and Functional Impact of the Rare S737F CFTR Variant and Its Responsiveness to CFTR ModulatorsInternational Journal of Molecular SciencesMarch 31, 2023Vito Terlizzi, Emanuela Pesce, Valeria Capurro, Valeria Tomati, Mariateresa Lena, Cristina Pastorino, Renata Bocciardi, Federico Zara, Claudia Centrone, Giovanni Taccetti, et al.nasal cells were seeded at high density (500,000 cells/cm2) on porous membranes (Snapwell inserts, code 3801, Corning Life Sciences, Corning, NY, USA). After 24 h, the serum-free medium was removed and replaced

(4) Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-AREFrontiers in Cell and Developmental BiologyDecember 7, 2022Lisette Sandoval, Mariana Labarca, Claudio Retamal, Paula Sánchez, Juan Larraín, Alfonso Gonzálezmm Transwells,Costar,3401,For immunofluorescence Polarized culture chamber,12 mm Snapwells,Costar,3801,For microinjection Key resources table.

(5) Gene-specific nonsense-mediated mRNA decay targeting for cystic fibrosis therapyNature CommunicationsDecember 1, 2022Young Jin Kim, Tomoki Nomakuchi, Foteini Papaleonidopoulou, Lucia Yang, Qian Zhang, Adrian R. Krainerassay 16HBEge cells were grown as an electrically tight monolayer on Snapwell filter supports (Corning, cat# 3801), as described68. In brief, the 16HBEge cells were seeded on Snapwell filter supports at a density of

(6) Natural statin derivatives as potential therapy to reduce intestinal fluid loss in choleraPLoS neglected tropical diseasesDecember 1, 2022Noitem R, Pongkorpsakol P, Changsen C, Sukpondma Y, Tansakul C, Rukachaisirikul V, Muanprasat C.Electrophysiological analyses T84 cells were seeded (5 x 105 cells/well) on Snapwell inserts (Cat#3801; Corning, Inc., 0.4 μm pore polyester membrane) and cultured for 10 days (TER >1,000 Ω cm2). Snapwell

(7) Vasoactive intestinal peptide and cystic fibrosis transmembrane conductance regulator contribute to the transepithelial calcium transport across intestinal epithelium-like Caco-2 monolayerPLoS OneNovember 18, 2022Mayuree Rodrat, Kannikar Wongdee, Jarinthorn Teerapornpuntakit, Jirawan Thongbunchoo, Duangrudee Tanramluk, Ratchaneevan Aeimlapa, Nithipak Thammayon, Natchayaporn Thonapan, Pathnaree Wattano, Narattaphol Charoenphandhuprepared by seeding cells on polyester Snapwell with 12-mm diameter and 0.4-μm pore size (catalog no. 3801; Corning) at 4.2 × 105 cells/well. Culture medium was changed after 48-h seeding. Monolayers were incubated

(8) In vivo correction of cystic fibrosis mediated by PNA nanoparticlesScience AdvancesOctober 7, 2022Alexandra S. Piotrowski-Daspit, Christina Barone, Chun-Yu Lin, Yanxiang Deng, Douglas Wu, Thomas C. Binns, Emily Xu, Adele S. Ricciardi, Rachael Putman, Alannah Garrison, et al.~1.5 × 106 cells were seeded on permeable polycarbonate supports with 0.4-mm pore size (Corning Costar 3801) in 250 ml of cell culture medium. These six-well plate inserts had a diameter of 12 mm and were coated

(9) The CFTR Amplifier Nesolicaftor Rescues TGF-β1 Inhibition of Modulator-Corrected F508del CFTR FunctionInternational journal of molecular sciencesSeptember 19, 2022Bengtson C, Silswal N, Baumlin N, Yoshida M, Dennis J, Yerrathota S, Kim M, Salathe M.seeded on Transwell inserts (Cat. No. 3460; Corning, Corning, NY, USA) or Snapwell inserts (Cat. No. 3801; Corning, Corning, NY, USA) at a density of 2 × 105 cells in ALI media. ALI media was prepared according

(10) Downstream Alternate Start Site Allows N-Terminal Nonsense Variants to Escape NMD and Results in Functional Recovery by Readthrough and Modulator CombinationJournal of Personalized MedicineSeptember 1, 2022Alyssa Bowling, Alice Eastman, Christian Merlo, Gabrielle Lin, Natalie West, Shivani Patel, Garry Cutting, Neeraj Sharmafor multiple passages. NE cells were then seeded onto snapwell filters (Costar, Kennebunk, ME, USA; #3801) coated with collagen type IV (~300,000 cells/filter). Upon confluency (5–7 days), media was changed

(11) Fe3+ opposes the 1,25(OH)2D3-induced calcium transport across intestinal epithelium-like Caco-2 monolayer in the presence or absence of ascorbic acidPLOS ONEAugust 30, 2022Sukpapohn Phummisutthigoon, Kornkamon Lertsuwan, Nattapon Panupinthu, Ratchaneevan Aeimlapa, Jarinthorn Teerapornpuntakit, Wasutorn Chankamngoen, Jirawan Thongbunchoo, Narattaphol Charoenphandhu, Kannikar Wongdeeporous polyester membrane, i.e., Snapwell with a diameter of 12 mm and pore size of 0.4 μm (catalog no. 3801; Corning), as reported previously [26]. Culture media was changed daily, and monolayers were incubated

(12) CFTR Rescue by Lumacaftor (VX-809) Induces an Extensive Reorganization of Mitochondria in the Cystic Fibrosis Bronchial EpitheliumCellsJune 16, 2022Braccia C, Christopher JA, Crook OM, Breckels LM, Queiroz RML, Liessi N, Tomati V, Capurro V, Bandiera T, Baldassari S, et al.were seeded at high density on porous membranes (Snapwell inserts, Corning, Corning, NY, USA, code 3801). After 24 h, the serum-free medium was removed from both sides and, on the basolateral side only, replaced

(13) Cell function and identity revealed by comparative scRNA-seq analysis in human nasal, bronchial and epididymis epitheliaEuropean Journal of Cell BiologyJune 1, 2022Alekh Paranjapye, Shih-Hsing Leir, Felix Huang, Jenny L. Kerschner, Ann Harrisgentamicin, and fungizone). Harvested cells were seeded (P2) onto collagen-coated 12 mm inserts (Costar 3801) in AW#2 with RI at 4 × 105 cells per insert. Media was replaced with AW#2 without ROCKi 24 h after

(14) Targeting the E1 ubiquitin-activating enzyme (UBA1) improves elexacaftor/tezacaftor/ivacaftor efficacy towards F508del and rare misfolded CFTR mutantsCellular and Molecular Life SciencesApril 1, 2022Christian Borgo, Claudio D’Amore, Valeria Capurro, Valeria Tomati, Elvira Sondo, Federico Cresta, Carlo Castellani, Nicoletta Pedemonte, Mauro Salviwere seeded at high density (500.000 cells/cm2) on porous membranes (Snapwell inserts, Corning, code 3801). After 24 h, the serum-free medium was removed from both sides and, on the basolateral side only, replaced

(15) A small molecule CFTR potentiator restores ATP‐dependent channel gating to the cystic fibrosis mutant G551D‐CFTRBritish Journal of PharmacologyApril 1, 2022Jia Liu, Allison P. Berg, Yiting Wang, Walailak Jantarajit, Katy J. Sutcliffe, Edward B. Stevens, Lishuang Cao, Marko J. Pregel, David N. Sheppardhuman bronchial epithelial cells were seeded in growth medium on Snapwell filter inserts (cat. no. 3801, Corning, NY, USA). Cells were switched to differentiation medium the following day and cultured submerged

(16) The L467F-F508del Complex Allele Hampers Pharmacological Rescue of Mutant CFTR by Elexacaftor/Tezacaftor/Ivacaftor in Cystic Fibrosis Patients: The Value of the Ex Vivo Nasal Epithelial Model to Address Non-Responders to CFTR-Modulating DrugsInternational Journal of Molecular SciencesMarch 15, 2022Elvira Sondo et al.nasal cells were seeded at high density (500,000 cells/cm2) on porous membranes (Snapwell inserts, code 3801, Corning Life Sciences, Corning, NY, USA). After 24 h, the serum-free medium was removed from both sides

(17) Expression of SLC26A9 in Airways and Its Potential Role in AsthmaInternational journal of molecular sciencesMarch 10, 2022Ousingsawat J, Centeio R, Schreiber R, Kunzelmann K.Switzerland). For polarization, BCi-NS1 cells were seeded onto permeable supports for up to 30 days (Snapwell #3801, Corning, New York, USA) in an air–liquid (ALI) interface. All cells were cultured at 37 °C in a humidified

(18) Journey on VX-809-Based Hybrid Derivatives towards Drug-like F508del-CFTR Correctors: From Molecular Modeling to Chemical Synthesis and Biological AssaysPharmaceuticals (Basel)February 23, 2022Alice Parodi et al.at high density on porous membranes (Snapwell inserts, Corning Life Sciences, Acton, MA, USA, code 3801). The following day, the serum-free medium was removed from both sides and, on the basolateral side

(19) Exon-skipping antisense oligonucleotides for cystic fibrosis therapyProceedings of the National Academy of SciencesJanuary 18, 2022Young Jin Kim, Nicole Sivetz, Jessica Layne, Dillon M. Voss, Lucia Yang, Qian Zhang, Adrian R. KrainerAssay. The 16HBEge cells were grown as an electrically tight monolayer on Snapwell filter supports (Corning, 3801), as described (38), and both serosal and mucosal membranes were exposed to the ASOs for 4 d and to

(20) Esc peptides as novel potentiators of defective cystic fibrosis transmembrane conductance regulator: an unprecedented property of antimicrobial peptidesCellular and Molecular Life SciencesJanuary 1, 2022Loretta Ferrera, Floriana Cappiello, Maria Rosa Loffredo, Elena Puglisi, Bruno Casciaro, Bruno Botta, Luis J. V. Galietta, Mattia Mori, Maria Luisa MangoniRecordings) Primary human bronchial epithelial were seeded on porous membranes (12 mm Snapwell inserts, Corning, code 3801) as previously described [68] to form a differentiated epithelium under air–liquid condition. Epithelia

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12 mm Snapwell™ Insert with 0.4 µm Pore Polyester Membrane, Sterile from Corning Life Sciences

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12 mm Snapwell™ Insert with 0.4 µm Pore Polyester Membrane, Sterile from Corning Life Sciences

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