Fig 1: Immunohistochemical analysis of biofilm exposed RHG. (a) Histology of healthy native gingiva. Histology (hematoxylin and eosin staining, H&E) and immunohistochemical staining with antibodies against PCNA, Ki67, Elafin and HBD-2 is shown. (b) RHG were exposed topically (surface application) to either multi-species biofilm or control medium without biofilm and harvested 1 or 7 days hereafter. Histology (H & E) shows thicker epithelium at day 7 in RHG exposed to biofilm compared to control RHG. Immunohistochemistry shows increased proliferation (PCNA, Ki67), Elafin and HBD-2 in biofilm exposed RHG compared to control RHG at day 7. Figures are representative of at least three independent experiments, each with an intra-experiment duplicate.
Fig 2: Gene expression of antimicrobial peptides. Cas9 and NLRP3-deficient (NLRP3 gRNA) bladder epithelial cells were stimulated with 1 nM or 10 nM estradiol for 24 h, followed by analysis of DEFB1 (A), DEFB4A (B), CAMP (C), Lactoferrin (D), RNASE6 (E), RNASE7 (F) mRNA expression. The gene expression was normalized to GAPDH and expressed as fold change relative to unstimulated Cas9 controls. Data are presented as mean ± SEM of three independent experiments. Asterisks show statistical significance compared to unstimulated control (* p < 0.05, ** p < 0.01, *** p < 0.001).
Supplier Page from OriGene Technologies for BD2 (DEFB4A) Human qPCR Primer Pair (NM_004942)