Fig 1: (a) Schematic showing the NSCLC cells cocultured with macrophages of different OPN levels in a transwell apparatus of 0.4 μm pore size; (b, c, d and e) A549 and H520 cells were treated with helenalin (Abcam, ab146197, 10 ng/ml) for 1 h or monoclonal anti‐rabbit OPN antibody, and then were cocultured with macrophages (RAW264.7‐Ctl or RAW264.7‐OPN) for 24 h. Western blot analysis of PD‐L1 expression in different cococulture groups
Supplier Page from Abcam for Helenalin, NF-kappaB inhibitor