Highly Satisfied With This Chicken Anti GFAP Antibody From Abcam

April 20, 2024

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-GFAP Antibody

Catalog Number:

ab134436

Clone Number:

N/A

The specific goal of this experiment was to assess astrocyte activation in mouse brain tissues. This antibody provided robust signals by immunofluorescence and enabled us to evaluate astrocyte activation in situ in tissues. This antibody host is chicken, which made multiplex with other antibodies possible.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:2000 at 4 degrees celsius for overnight

Blocking Agent

10% normal donkey serum

Secondary Incubation

Alexa fluor anti-chicken IgG at 1:500 for overnight at room temp

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Very robust and specific immunoreactivity in astrocytes was observed by immunofluorescence with this antibody in mouse brain tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very robust signal.

The Bad

None.

The Bottom Line

This chicken anti-GFAP antibody is highly recommended especially when researchers plan to do multiplex immunofluorescence experiments.

Share your experience with other scientists. Write a Review! »

Join the discussion