Reliable GFAP Antibody for Astrocytes in the Mouse Retina

April 19, 2024

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody

Catalog Number:

G3893

Clone Number:

G-A-5

This antibody was used in a study that investigated astrocyte pathology in the retina. This GFAP antibody has provided the best signal/background ratio among GFAP antibodies tested so far.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse retina

Primary Incubation

1:50 at 4 degrees celsius for overnight

Blocking Agent

10% normal donkey serum

Secondary Incubation

Alexa fluor anti-mouse IgG at 1:500 for 1hr at room temp

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

The antibody worked excellently for mouse retina sections, with minimal background staining compared to isotype control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very specific.

The Bad

None.

The Bottom Line

This is a very good antibody to detect GFAP in astrocytes.

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