At the heart of immunohistochemistry (IHC) lies the ability to not just detect specific antigens detect their location in tissue with some degree of precision. Though it sounds straightforward on its surface, IHC is a technique fraught with opportunities to falter.
Many criteria must be considered for successful IHC to work including: preparing a specimen, selecting the reagents such as antibodies, buffers for blocking and washing the sample, optimizing the protocol, and using the best detection method to see your results.
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